Transfer Membranes
Selecting the appropriate membrane is critical to the success of protein transfer and nucleic acid procedures. Using the correct protocol with Osmonics membranes insures you that your procedure will perform with optimal results.
Osmonics provides the following tried and proven protocols developed by MSI to obtain consistent reproducible and dependable results when used with Osmonics membranes.
Technical Papers
- Transfer Membranes Manufactured, Tested, and Stocked by Osmonics
Explore the range of transfer membranes manufactured by Osmonics
- Osmonics Transfer Membranes by Application
You know you your application, we know the membrane you require
- Osmonics Hybridization Membrane Trade Name Conversions
Find the correct Osmonics filter to cross-reference with competitor part numbers
- Selecting a Transfer Membrane
Procedures and features of hybridization membranes plus troubleshooting guide.
- Recipes for Buffers used in Nucleic Acid Transfers
Learn the secrets to mixing your own buffers
- Non-Radioactive Detection Systems with Magna Nylon or MagnaGraph
Procedure for non-radioactive systems - MagnaProbe Nylon may be used particularily where multiple probing is involved.
- Non-Radioactive Detection Systems
Use non-radioactive probes for hybridizations with MagnaProbe, Magna Nylon or MagnaGraph Nylon membranes
- Southern (DNA) and Northern (RNA) Hybridizations with MagnaProbe Nylon Membranes
Southern (DNA) and northern (RNA) protocol for our charged nylon - MagnaProbe - a membrane manufactured for your multi-probe needs.
- Southern (DNA) and Northern (RNA) Hybridizations
Run Southern DNA and Northern (RNA) Hybridizations with MAGNA and MAGNACHARGE nylon membranes
- Southern (DNA) and Northern (RNA) Hybridizations
Run Southern DNA and Northern (RNA) Hybridizations with MAGNACHARGE
- Southern (DNA) and Northern (RNA) Hybridizations
Protocol for use with NitroPure, NitroBind or NitroPlus nitrocellulose membranes.
- RNA Probes to be Used in Northern and Southern Blotting
Use MAGNA and MAGNACHARGE and MagnaGraph nylon in northern and southern blotting
- Oligonucleotide Probes to be Used in Northern and Southern Blotting
Use MAGNA and MAGNACHARGE and MagnaGraph nylon membranes in Northern and Southern Blotting
- Non-Isotopic Hybridization and Detection Using Biotinylated Probes with GE Osmonics MagnaGraph Nylon Transfer Membrane
How to use MagnaGraph Nylon transfer membranes in non-isotopic hybridizations
- Alkaline Blotting with MagnaProbe
Membranes with very high binding capacities are necessary for the success alkaline blotting - like MagnaCharge and MagnaProbe membranes
- Colony Hybridization and Plaque Lifts with MAGNA Nylon Membranes
Perform colony hybridizations and plaque lifts with MAGNA LIFT Nylon Membranes
- Colony Hybridizations and Plaque Lifts with Magna and MagnaProbeNylon Membranes
Perform colony hybridizations and plaque lifts with MAGNA Nylon Membranes
- Colony Hybridizations and Plaque Lifts with Nitrocellulose Transfer membranes
Perform colony hybridizations and plaque lifts with NitroPure, NitroBind and NitroPlus Nitrocellulose membranes
- Nucleic Acid Dot/Slot Blotting
Use MAGNACHARGE Nylon membranes to purify DNA or RNA
- Nucleic Acid Dot/Slot Blotting
Purify DNA or RNA with Magna and MagnaGraph Nylon membranes
- Nucleic Acid Dot/Slot Blotting
Purify DNA or RNA with NitroPure, NitroBind or NitroPlus Nitrocellulose membranes
- Western (Protein) Blotting
For use with NitroPure, NitroBind and NitroPlus Membranes
- Western Blotting from a SDS-PAGE System
Use PVDF-Plus transfer membranes for Western Blotting from a SDS-PAGE System
- Protein Sequencing From A Western Blot
Use MSI PVDF-Plus for protein sequencing from a Western Blot
- Stripping Magna PVDF (Polyvinylidene Fluoride) Transfer Membrane
A stripping procedure that will not adversely alter membrane bound proteins
- Transfer of High Molecular Weight Proteins from a Non-Denaturing Gel System to the Magna PVDF (Polyvinylidene Fluoride) Membrane
Use PVDF-PLUS transfer membranes in non-denaturing gel systems
- Transfer of Basic Proteins from an Acid/Urea Gel System at Acidic pH to the Magna PVDF (Polyvinylidene Fluoride) Transfer Membrane
Use PVDF-PLUS transfer membranes as an alternative to nitrocellulose in Western Blotting applications
- Protein Dot Slot Blotting with PVDF (Polyvinylidene Fluoride)Transfer Membranes
Perform Dot Slot Blotting with MSI PVDF-PLUS transfer membranes