PVDF Transfer Membrane 0.45um 30cm X 3m Roll
Product ID: PV4HY00010
Polyvinylidene Flouride (PVDF) Transfer Membranes. Size: 300mm x 3m, 0.45µm. 1 Roll.
The membranes are designed, manufactured and tested specifically for RNA, DNA and protein analysis providing molecular biologists with high quality materials for outstanding results, at exceptionally economical prices.
PVDF is a naturally hydrophobic, unsupported polyvinylidene fluoride transfer membrane designed, manufactured and tested by GE to deliver the highest binding capacity with low backgrounds in Western transfer, amino acid analysis and protein sequencing applications. Exceptional quality control procedures throughout the manufacturing process eliminate “lot-to-lot” variation and greatly minimize “bald spot” problems. PVDF assures reproducible results with maximum sensitivity.
Performance
Protein can be electroblotted from a variety of gel matrices onto Osmonics PVDF. Because of its high sensitivity and strong binding affinity, protein will not pass through the membrane. The broad chemical compatibility of Osmonics PVDF allows the use of all commonly used stains such as: Amido Black, Colloidal Gold, Coomassie Blue, India Ink and Ponceau-S. PVDF will not degrade, distort or shrink when using high concentrations of methanol for distaining. The exceptional tensile strength allows for multiple re-probing and easy removal of target bands without concern for the membrane tearing, fracturing or curling.
Osmonics PVDF exhibits efficient transfer of proteins in a wide range of molecular weights, from 5kD to 700kD. The binding capacity average 125μg/cm² for larger globular proteins such as immunoglobulins and higher binding capacities for small peptides meaning efficient transfer every time
Specifications
·Naturally hydrophobic unsupported membrane
·Chemical compatibility allows the use of all commonly used stains
·Low backgrounds, high sensitivity
·Highest binding capacity eliminating sample “blow through”
·BSA binding capacity is 125μg/cm²
Applications
·Western transfers
·Protein sequencing
·Peptide mapping
·Amino acid analysis
·Dot/slot blots (please enquire for size)
